CURRICULUM VITAE
JOHN N. VOURNAKIS, PH.D.

 

PUBLICATIONS & BIBLIOGRAPHY

More than 100 publications have appeared including journal articles, abstracts, chapters and books. In addition, twenty seven patents have issued and several others have been filed and are in process.

 

A. ISSUED PATENTS:

1.

Bouriotis, V., Kafetzopoulos, D. and Vournakis, J.N.: US Patent# 5,219,749, “Process for Preparing Purified Chitin Deacetylase”, issued June 15, 1997.

 

 

 2.

Vournakis, J., Pariser, E.R., Finkielsztein, S. and Helton, M.: US Patent # 5,623,064, “Poly-N-acetyl Glucosamine”, issued April 22, 1997.

 

 

3.

Vournakis, J., Pariser, E.R., Finkielsztein, S. and Helton, M.: US Patent # 5,622,834, “Method of Isolating Poly-N-acetyl Glucosamine from Microalgal Culture”, issued April 22, 1997.

 

 

4.

Vournakis, J., Pariser, E.R., Finkielsztein, S. and Helton, M.: US Patent # 5,624,679, “Methods and Compositions for Poly-N-acetyl Glucosamine Biological Barriers”, issued April 29, 1997.

 

 

5.

Vournakis, J., Pariser, E.R., Finkielsztein, S. and Helton, M.: US Patent # 5,635,493, “Methods and Compositions for Poly-N-acetyl Glucosamine Chemotherapeutics”, issued June 3, 1997. 

 

 

6.

Vournakis, J., Pariser, E.R., Finkielsztein, S. and Helton, M.: US Patent # 5,686,115, “Poly-N-acetyl Glucosamine Copolymer Compositions with Collagen”, issued November 11, 1997. 

 

 

7.

Vournakis, J., Pariser, E.R., Finkielsztein, S. and Helton, M.: New Zealand Patent    # 277662, “Poly-N-acetyl Glucosamine”, issued August 17, 1998.

 

 

8.

Vournakis, J., Pariser, E.R., Finkielsztein, S. and Helton, M.: US Patent #5,846,952, “Methods & Compositions for Poly-N-acetyl Glucosamine Drug Delivery”, issued December 8, 1998.

 

 

9.

Vournakis, J., Pariser, E.R., Finkielsztein, S. and Helton, M Australian Patent # 695850, “Poly-N-acetyl Glucosamine”, issued December 10, 1998.

 

 

10.

Vournakis, J., Pariser, E.R., Finkielsztein, S. and Helton, M.: US Patent #5,858,350, “Methods & Compositions for Poly-N-acetyl Glucosamine Cell Therapy System”, issued January 12, 1999. Vournakis, J., Finkielsztein, S. and Pariser, E.R: US Patent #6,063,911, “Methods and Compositions for   Treatment of Cell Proliferative Disorders”, issued March 16, 2000.

   

11.

Vournakis, J., Finkielsztein, S. and Pariser, E.R: US Patent #6,063,911, “Methods and Compositions for Treatment of Cell Proliferative Disorders”, issued March 16, 2000.

   

12.

Pavlakis, G., Gaitanaris, G., Stauber, R. and Vournakis, J.: US Patent#6,027,881, “Mutant Aequorea Victoria Fluorescent Proteins Having Increased Specific Fluorescence”, issued February, 2000. 

 

 

13.

Pavlakis, G., Gaitanaris, G., Stauber, R. and Vournakis, J.: Australian Patent #734239, “Mutant Aequorea Victoria Flourescent Proteins having Increased Cellular Fluorescence”, issued June, 2001. 

 

 

14.

Pavlakis, G., Gaitanaris, G., Stauber, R. and Vournakis, J.: US Patent #6,265,548, “Mutant Aequorea Victoria Flourescent Proteins having Increased Cellular Fluorescence”, issued July, 2001. 

 

 

15.

Vournakis, J.N., Seth, A.K. and Papas, T.S.: New Zealand Patent #338025, “Methods and Compositions for Diagnosis and Treatment of Breast Cancer”, issued October 9, 2001. 

 

 

16.

Vournakis, J.N., Seth, A.K. and Papas, T.S.: US Patent # 6,399,328, “Methods and Compositions for Diagnosis and Treatment of Breast Cancer”, issued March 20, 2002. 

 

 

17.

Vournakis, J.N., Seth, A.K. and Papas, T.S.: Australian Patent #67685/98,“Methods and Compositions for Diagnosis and Treatment of Breast Cancer”, accepted August 15, 2002. 

 

 

18.

Vournakis, J., Pariser, E.R., Finkielsztein, S. and Helton, M.: US Patent #6,599,720, “Methods for making Poly-N-acetyl Glucosamine”, issued July 29, 2003. 

 

 

19.

Vournakis, J., Pariser, E.R., Finkielsztein, S. and Helton, M.: US Patent #6,610,668, “Methods and composition for Poly-N-acetyl Glucosamine cell therapy system”, issued August 26, 2003.

 

 

20.

Vournakis, J., Pariser, E.R., Finkielsztein, S. and Helton, M.: US Patent #6,630,459, “Pharmaceutical compositions comprising Poly-N-acetyl Glucosamine”, issued October 7, 2003.

 

 

21.

Vournakis, J., Pariser, E.R., Finkielsztein, S. and Helton, M.: US Patent #6,649,599, “Methods and compositions for poly-.beta.-1-4-N-acetylglucosamine cell therapy system”, issued November 18, 2003.

 

 

22.

Vournakis, J., Pariser, E.R., Finkielsztein, S. and Helton, M.: US Patent #6,686,342, “Biocompatible poly-.beta.-1-4-N-acetylglucosamine”, issued February 3, 2004. 

 

 

23.

Vournakis, J., Pariser, E.R., Finkielsztein, S. and Helton, M.: US Patent #6,743,783, “Pharmaceutical compositions comprising poly-.beta.-1.fwdarw.4-N-acetylglucosamine”, issued June 1, 2004.

 

 

24.

Vournakis, J., Pariser, E.R., Finkielsztein, S. and Helton, M.: US Patent #6,864,245 “Biocompatible poly-.beta.-1.fwdarw.4-N-acetylglucosamine”, issued March 8, 2005.

 

 

25.

Vournakis, J. and Finkielsztein, S.: US Patent #7,041,657 “Compositions and methods for modulation of vascular structure and/or function”, issued May 9, 2006.

 

 

26.

Vournakis, J. and Finkielsztein, S.: US Patent #7,115,558 “Methods for treating a breach or puncture in a blood vessel”, issued October 3, 2006.

 

 

27.

Vournakis, J. and Finkielsztein, S.: US Patent #7,285,266 “Cell-polymer fiber combinations and uses thereof”, issued October 23, 2007. 

   
   
B.  PATENTS FILED - IN PROCESS:

 

 

1.

Vournakis, J. and Finkielsztein, S.: “Hemostatic compositions and uses thereof”, December 4, 2004.
   

2.

Vournakis, J., Finkielsztein, S., Pariser, E.R. amd Helton, M.: “Methods and combinations for poly-beta-1-4-N-acetylglucosamine”, January 6, 2005.
   

3.

 

Vournakis, J., Finkielsztein, S. and Pariser, E.R.: “Vascular access preservation in hemodialysis patients”, April 7, 2005.
   

4.

Finkielsztein, S., Finkielsztein, M. and Vournakis, J.N.: “Methods and apparatus for a manual vascular compression device”, October 12, 2006.

   

5.

Vournakis, J.N. and Finkielsztein, S.: Cell-polymer fiber compositions and uses thereof”, January 31, 2008.

   

6.

Vournakis, J. and Finkielsztein, S.: “Hemostatic combinations and therapeutic regimens”, May 7, 2009.
   

7.

Vournakis, J.N.,  Finkielsztein, S., Pariser, E.R. and Helton, M.: “Biocompatible Poly-beta-1-4-N-acetylglucosamine”, October 1, 2009.
   

8.

Vournakis, J. and Finkielsztein, S.: “Composition and methods for modulation of vascular structure and/or fnction”, December 23, 2010.

   

9.

Orgill, D.P., Pietramaggiori, P., Finkielsztein, S. and Vournakis, J.N.: “Application of polymeric materials to facilitate hemostasis and wound healing”, January 20, 2011

   

10.

Pavlakis, G.N., Vournakis, J.N., Felber, B.K. and Finkielsztein, S.: “Complexes of IL-15 and IL-15Ra and uses therof.”, April 7, 2011.

   
   
   

C.  PUBLICATIONS: RESEARCH ARTICLES: 

 

1.

Vournakis JN, Scheraga HA, Rushisky GW.  Neighbor-neighbor interactions in single stranded polyribonucleotides:  Rotatory dispersion studies of the ribonucleotide ApApCp.  Biopolymers 1966; 4:  33. 

   

2.

Vournakis JN, Poland D, Scheraga HA.  Cooperative interactions in single strand oligomers of deoxyribonucleic acid.  Biopolymers 1966; 4:  223.

 

 

3.

Vournakis JN, Scheraga HA.  Optical rotatory dispersion studies of yeast alanine and tyrosine transfer DNA: Evidence for intramolecular hydrogen bonding and discussion of conformation aspects.  Biochemistry 1966; 5:  2997.

 

 

4.

Vournakis JN, Poland D, Scheraga HA.  Anti-cooperative interactions in single strand oligomers of deoxyribonucleic acid.  Biopolymers 1967; 5:  403.

 

 

5.

Vournakis JN, Yan J, Scheraga HA.  Effects of backbone conformation and side chain on the conformational energy and rotational strength of the n-p* transition for some a-helical polyamino acids.  Biopolymers 1969; 6:  1531.

 

 

6.

Low RB, Vournakis JN, Rich A.  Identification of separate polysomes active in the synthesis of the light and heavy chains of myosin.  Biochemistry 1971; 10:  1813.

 

 

7.

Vournakis JN, Rich A.  Size changes in eukaryotic ribosomes.  Proc. Nat. Acad. Sci. USA 1971; 68:  3021.

 

 

8.

Hocker LJ, Krugg GB, Vournakis JN.  Observations of self-aggregation and dissociation of E. coli ribosomes by optical mixing spectroscopy.  Biopolymers 1972; 12:  1677.

 

 

9.

Vournakis JN, Rich A.  Ribosomal transformations during protein synthesis.  In:  Cox RA, ed.  Functional Units in Protein Biosynthesis, Academic Press, 1972.

 

 

10.

Vournakis JN, Leick V, Rich A.  Ribosomal conformations and protein synthesis in eukaryotes.  In:  Cold Spring Harbor Symposium on Protein Synthesis, 1972.

 

 

11.

Barron M, Bromley A, Raw I, Pariser ER, Vournakis JN.  A new integrated science curriculum for freshmen based on nutrition.  AIBS Educ. Rev. 1974; 3:  9.

 

 

12.

Raw I, Bromley A, Holleman G, Pariser ER, Vournakis JN.  A simple, low cost calorimeter for measuring heat of combustion and carbon and hydrogen content.  J. Chem. Ed. 1974; 51:  959.

 

 

13.

Vournakis JN.  A non-competitive introductory organic chemistry course for pre-medical students.  J. Chem. Ed. 1974; 51:  742.

 

 

14.

Vournakis JN, Gelinas R, Kafatos FC.  Short polyadenylic acid sequences in insect chorion messenger RNA.  Cell 1974; 3:  267.

 

 

15.

Raw I, Bromley A, Pariser ER, Vournakis JN.  What people eat.  A chemistry program based on nutrition.  J. College Science Teaching 1975; 4:  177.

 

 

16.

Efstratiadis A, Maniatis T, Kafatos FC, Jeffrey A, Vournakis JN.  Full length and discrete partial reverse transcripts of globin and chorion mRNAs.  Cell 1975; 4:  367.

 

 

17.

Vournakis JN.  Purification and biophysical characterization of rabbit globin and silk-moth chorion protein messenger RNA.  Biophys. Jour. 1975; 15:  64.

 

 

18.

Vournakis JN, Efstradiadis A, Kafatos FC.  Electrophoretic patterns of deadenylated globin and chorion mRNAs.  Proc. Nat. Acad. Sci. USA 1975; 72:  2959.

 

 

19.

Raw I, Pariser ER, Vournakis JN.  What people eat.  In:  An Introduction to Chemistry and Food Science.  San Francisco:  W. T. Kaufmann, 1975.

 

 

20.

Kafatos FC, Vournakis JN, et al.  The eggshell of insects:  Differentiation specific proteins and the control of their synthesis and accumulation during development.  In:  Beerman W, ed.  Results and Problems in Cell Differentiation.  New York:  Springer-Verlag, Inc., 1975:  45-139 (vol 8).

 

 

21.

Vournakis JN, Carmichael GC, Efstratiadis A.  Synthesis of DNA complementary to rabbit globin mRNA by QB replicase.  Biochem. and Biophys. Res. Commun.  1976; 70:  74.

 

 

22.

Vournakis JN, Flashner M, Kitos G, Katopes J, Vamvakopoulos N, Sell M, Wurst R.  Structural studies on intact and deadenylated rabbit globin messenger RNA.  In:  Prog. Nucl. Acid Res. and Mol. Biol.  New York:  Academic Press, 1976:  223 (vol 19).

   

23.

Vournakis, J., Pariser, E.R., Finkielsztein, S. and Helton, M.: US Patent #6,864,245 “Biocompatible poly-.beta.-1.fwdarw.4-N-acetylglucosamine”, issued March 8, 2005.

 

 

24.

Kafatos FC,  Regier,JC, Mazur GD, Nadel MR, Blau HM, Petri WH, Wyman AR, Gelinas RE, Moore PB, Paul M, Efstratiadis A, Vournakis JN, Goldsmith MR, Hunsley JR, Baker B, Nardi J and Koelher M. The eggshell of insects: differentiation-specific proteins and the control of their synthesis and accumulation during development. In Results and Problems in Cell Differentiation. 1977; 8:45-145.

 

 

25.

Vamvokopoulos NC, Vournakis JN, Marcus SL.  The effect of magnesium and manganese ions on the structure and template activity for reverse transcriptase of polyribocytidylate and its 2'-0' methyl derivative.  Nucl. Acids Res.  1977; 4:  3589.

 

 

26.

Efstratiadis AE, Vournakis JN, et al.  End labeling of enzymatically decapped mRNA.  Nucl. Acid Res. 1977; 4:  4165.

 

 

27.

Wurst RM, Vournakis JN, Maxam AM.  Structure mapping of 5'[P]-labeled RNA with S1 nuclease.  Biochemistry 1978; 17:  4493.

 

 

28.

Wrede P, Wurst R, Vournakis JN, Rich A.  Conformational changes of yeast RNAPhe and E. coli tRNA as indicated by different nuclease digestion patterns.  J. Biol. Chem. 1979; 254:  9608.

 

 

29.

Pavlakis GN, Jordan BR, Wurst RM, Vournakis JN.  Sequence and structure of Drosophila melanogaster 5.8S and 2S rRNAs and the processing site between them.  Nucl. Acid Res. 1979; 7:  2213.

 

 

30.

Pavlakis GN, Lockard R, Vamvakopoulos NC, Rieser L, RajBhandary U, Vournakis JN.  Secondary structure of mouse and rabbit a and b globin mRNAs:  Differential accessibility towards nuclease of and initiator AUG condons.  Cell 1980; 19:  91.

 

 

31.

Wurst R, Vournakis JN.  Structural studies of tRNA fragments by partial digestion with S1 nuclease.  Biochemistry International 1981; 2:  585. 

   

32.

Vournakis JN, et al.  Sequence and structure analysis of end-labeled RNA with nucleases.  In:  Chirikjian J, Papas T, eds. Gene Amplification and Analysis, Vol. II.  Elsevier-North Holland, Inc., 1981:  267-297.

 

 

33.

Lockard R, Rieser L, Vournakis JN.  5'-32 P-end labeling of eukaryotic messenger RNA:  Use of tobacco acid pyrophosphatase for removal of cap structures.  In:  Chirikjian J, Papas T, eds.  Gene Amplification and Analysis, Vol. II.  Elsevier-North Holland, Inc., 1981:  267-297.

 

 

34.

Auron PE, Rindone WP, Vary CPH, Celentano JJ, Vournakis JN.  Computer-aided prediction of RNB secondary structures.  Nucl. Acids Res. 1982; 10:  403.

 

 

35.

Troutt A, Savin TJ, Curtiss W, Celentano JJ, Vournakis JN.  Secondary structure of Bombyx mori and Dictyostelium discoidem 5S rRNA from S1 nuclease and cobra venom nuclease susceptibility and computer aided analysis.  Nucl. Acids Res. 1982; 10:  653.

 

 

36.

Vary CPH, Vournakis JN.  Secondary structure of eukaryotic messenger RNA.  Biochem. Soc. Symp. 1982; 47:  61.

 

 

37.

Vournakis JN, Vary CPH.  Messenger RNA conformation and the regulation of protein synthesis.  In:  Safer B, Grunberg-Manago M, ed.  Transcriptional/Translational Regulation of Protein Synthesis.  New York:  Elsevier-North Holland, 1982:  205-280.

 

 

38.

Vournakis JN, Elander RP.  Genetic manipulation of antibiotic producing micro-organisms.  Science 1983; 219:  203.

 

 

39.

Vournakis JN, Elander RP.  Genetic manipulation of antibiotic producing micro-organisms.  Science 1983; 219:  203.

 

 

40.

Lane ME, Dabrowiak JD, Vournakis JN.  Sequence specificity of actinomycin D and netropsin binding to pBR322 DNA by DNase 1 directed protection analysis.  Proc. Nat. Acad. Sci. USA 1983; 8:  3260.

 

 

41.

Vary CPH, Vournakis JN.  RNase H catalyzed site specific deadenylylation of rabbit and globin mRNAs:  Application to the study of the secondary structure of 3'-non coding regions.  J. Biol. Chem.  1984; 259:  3299.

 

 

42.

Vournakis JN, Vary CPH.  The role of mRNA structure in the regulation of protein synthesis:  Implications for studies of development.  Developmental Genetics 1984; 4:  313.

 

 

43.

Vournakis, JN and Elander, RP. Genetic Manipulation of Antibiotic Producing Microorganisms. In: Abelson, PH, ed., Biotechnology & Biological Frontiers, AAAS Publication # 84-8, Washington, DC, 1984: 188-200.

 

 

44.

Elander, E.P. and Vournakis, JN. Genetic Aspects of Overproduction of Antibiotics and Other Metabolites. In: Vanek, Z., ed., Excessive Metabolites: Strategy of Superior Strain Design and Fermentation Processes. Reading Massachusetts: Addison Wesley, 1986:63-80 (biotechnology Series).

 

 

45.

Curtiss WC, Vournakis JN.  Quantitation of base substitutions in eukaryotic 5S rRNA:  Selection for maintenance of secondary structure.  J. Vol. Evol.  1984; 20:  351.

 

 

46.

Vary CPH, Vournakis JN.  RNA structure analysis using methidium-propyl EDTA:  A new base-pair specific RNA structure probe.  Proc. Nat. Acad. Sci. USA 1984; 8l:  6978.

 

 

47.

Vournakis JN.  Genetic engineering and recombinant DNA.  Syracuse Scholar, Syracuse, New York 1984:  57-65.

 

 

48.

Vary, C.P.H. and Vournakis, JN RNAStructure Analysis using T2 Ribonuclease: detection of pH and metal ion induced conformational changes in yeast tRNA-Phe. Nucl. Acids Res. 1984; 12:6763.

 

 

49.

Lane MJ, Vournakis JN, Dabrowiak JD.  Footprinting analysis as a means of quantitating anti-tumor drug-DNA interactions.  In:  Rein R, ed.  Molecular Basis of Cancer.  New York:  Alan R. Liss, Inc., 1985:  145-153.

 

 

50.

Vary CPH, Lane MJ, Dabrowiak JD, Vournakis JN.  Quantitative microdensitometric scanning of footprinting autoradiograms.  In:  Neuhoff V, ed.  Electrophoresis 84.  Basel:  Verlag Chromie, 1984:  312-315. 

 

 

51.

Vournakis JN.  Role of mRNA structure in the regulation of protein synthesis:  Implications of mRNA engineering.  Am. Soc. of Microbiology, Washington, DC; Microbiology-1985; 1985:  473-479.

 

 

52.

Vary CPH, Vournakis JN.  The use of acrylamide-agarose composite gels to measure relative rates of initiation of protein systhesis by eukaryotic mRNAs.  Gene Anal. Tech.  1985; 2:  50.

 

 

53.

Dabrowiak JC, Skorobogaty A, Rich N, Vary PH, Vournakis JN.  Computer assisted microdensitometric analysis of footprinting autoradiographic data.  Nucl. Acids Res. 1986; 14:  489.

 

 

54.

Sneath B, Vary C, Pavlakis G, Vournakis JN.  Secondary structue of Tetrahymena thermophilla 5S ribosomal RNA as revealed by enzymatic digestion and microdensitometric analysis.  Nucl. Acids Res.  1986; 14:  1365.

 

 

55.

Vournakis JN.  Prevention of cancer:  Current considerations.  New Dev. in Clin. Med.  1986; 3:  25.

 

 

56.

Psarianos CG, Maramaras VL, Vournakis JN.  Tyrosine-4-0-8 glucoside in the Mediterranean fruit-fly Ceratitis capitata.  Insect Biochem. 1985; 15:  129.
   

57.

Graziano SL, Vournakis JN, Poiesz BJ, et al.  Quantitative assay of human T-cell leukemia/lymphoma virus transformation.  Cancer Research 1987; 47:  2468. 

   

58.

Fish EL, Vournakis JN.  Properties of DNase I digestion of the deoxyoligodecamer:  d(ATCGTACGAT).  Nucleic Acids Res. 1987; 15:  9417.

 

 

59.

Fish EL, Lane MJ, Vournakis JN.  Determination of equilibrium binding affinity of distamycin and netropsin to the synthetic deoxyoligonucleotide sequence d(GGTATACC), by quantitative DNase I footpringing.  Biochemistry 1988; 27:  6026.

 

 

60.

Vournakis JN, Runstadler PW Jr.  Microenvironment:  The key to improved cell culture products.  Biotechnology 1989; 7:  143.

 

 

61.

Ray NG, Tung AS, Hayman EG, Vournakis JN, Runstadler PW.  Continuous cell culture in fluidized bed bioreactors:  Cultivation of hybridomas and recombinant CHO cells immobilized in collagen microspheres.  Academy of Sciences:  Biomedical Engineering VI 1989:  443-457.

 

 

62.

Vournakis JN, Hayman EG, Ray NG, Tung AS.  Production of recombinant proteins and monoclonal antibodies in continuous immobilized cell culture.  In:  Spier R, Griffiths B, eds.  Advances in Animal Cell Biology and Technology for Bioprocesses.  Stoneham, Massachusetts:  Butterworths Publisher, 1989:  53-65.

 

 

63.

Vournakis, JN, Hayman, E.G.  Cell Microenvironment & the Large-scale Culture of Recombinant Chinese Hamster Ovary (CHO) Cells: Attachment of Cells to fibronectin in Collagen Microspheres and Formation of an Extracellular Matrix.  Verax Corporation Technical Bulletin, December, 1989.

 

 

64.

Vournakis JN, Runstadler PW Jr.  Optimization of the microenvironment for mammalian cell culture in flexible collagen microspheres in a fluidized bed bioreactor.  Verax Corporation Technical Memorandum 1990; 250:  1-25.

 

 

65.

Ray NG, Tung AS, Vournakis JN, Runstadler PW.  Optimization of culture productivity in fluidized-bed bioreactors:  Effect of perfusion rate.  In:  Proceedings of Interphex USA '90, eds., 1990.

 

 

66.

Vournakis, JN, Ray, N.G., Tung, A.S.  Cell Microenvironment and the Large-scale culture of Recombinant Hamster Ovary (CHO) Cells: Increase in Cell Density and Specific Protein Productivity by Culturing Cells with Microspheres in Bioreactors in Serum-free Medium. Verax Corporation Technical Bulletin, September, 1990.

 

 

67.

Ray NG, Vournakis JN, Runstadler PW, Tung AS., Venkatasubramanian K.  Physiology of hybridoma and recombinant chinese hamster ovary cells immobilized in collagen matrix.  In:  deBont JAM, Visser J, Mattiasson B, Tramper J, eds.  Physiology of Immobilized Cells.  Amsterdam:  Elsevier Science Publishers B.V., 1990:  699-711.

 

 

68.

Ray NG, Tung AS, Runstadler PW, Vournakis JN.  Enhanced productivity of hybridoma and recombinant CHO cell cultures by plutronic perfusion rates in fluidized-bed bioreactors.  In:  SpieR, Griffiths B, eds.  Production of Biologicals from Animal Cells in Culture:  Research Development and Achievements, Proceedings of 10th ESACT Meeting.  Stoneham, Massachusetts: Butterworths Publishers, 1990:  502-511.

 

 

69.

Ray NG, Tung AS, Runstadler PW, Vournakis JN.  Enhanced productivity of hybridoma and recombinant CHO cell cultures by plutronic perfusion rates in fluidized-bed bioreactors. Verax Technical Memo # 278; 1990.

 

 

70.

Vournakis JN, Runstadler PW.  Optimization of the microenvironment of mammalian cell culture in flexible collagen microspheres in a fluidized-bed bioreactor.  In:  Ho C, Wang D, eds.  Animal Cell Bioreactors.  Stoneham, Massachusetts:  Butterworths Publishers, 1990:  305-326.

 

 

71.

Venkatasubramanian, K, Runstadlaer, PW, Vournakis, JV and Ray, NG New Reactors for Animal Cell Cultures. In: Hollenberg, CP and Sahm, H eds, Biotec 3, Therapeutics, Diagnostics, Cell Cultures and Product Isolation. New York, Stuttgart, Gustav Fischer Press, 1990: 91-104.

 

 

72.

Huckaby CS, Vournakis JN.  An efficient technique for obtaining sequences flanking inserted retroviruses.  Gene Analysis:  Techniques and Applications 1991; 8 (5):  152.

 

 

73.

Tung AS, Ray NG, Corace RA, Vournakis JN, Runstadler PW.  Mammalian cell culture:  Making it work.  In:  Olson WP, Groves MJ, eds.  Sterile Pharmaceutical Mfg. Technology for the 1990's.  Prairie View, Illinois:  Interpharm Press, Inc., 1991:  43-92 (vol 3).

 

 

74.

Kafetzopoulos D, Bouriotis V, Thireos G, Vournakis J.  The primary structure of a fungal chitin deacetylase reveals the function of two bacterial gene products.  PNAS (USA) 1993; 90:  8005.

 

 

75.

Kafetzopoulos D, Bouriotis V, Thireos G, Vournakis J.  cDNA cloning of chitin deacetylase from Mucor rouxii.  In:  Muzzarelli RAA, ed.  Chitin Enzymology.  Ancona:  European Chitin Society, 1993:  431-436.

 

 

76.

Vournakis JN, Pariser ER, Finkielsztein S, Parker S, Helton MJ.  Isolation and characterization of pure Poly-N-Acetyl glucosamine:  Controlled enzymatic deacetylation and formulation for tissue engineering applications. J. Cell. Biochem.  1994; 18C:  283.

 

 

77.

Papas, T.S., Bhat, N.K., Spyropoulos, D.M., Mjaavedt, A., Vournakis, JN, Seth, A. and Watson, D.K.: Functional Relationships Amomg ETS Gene Family Members. Leukemia 11:557-566, 1997.

 

 

78.

Cole, D., Gattoni-Celli, S., McClay, E., Metcalf, J. Brown, J. Nabavi, N., Newton, D., Woolhiser, C., Wilson, M. and Vournakis, JN: Characterization of a Sustained Release Delivery System for Combined Cytokine/Peptide Vaccination Using a Poly-N-acetyl Glucosamine Based Polymer Matrix.  Clinical Cancer Research 3: 867-873, 1997.

 

 

79.

Burger, A., Li, H., Zhang, X., Pienkowska, M., Venanzoni, M., Vournakis, J., Papas, T. and Seth, A.: Breast Cancer Genome Anatomy: Correlation of Morphological Changes in Breast Carcinomas with Expression of the Novel Gene Product Di12. Cancer Research, Oncogene 16: 327-  333, 1998.

 

 

80.

Kulling, D., Woo, S., Demcheva, M., Hawes, R. and Vournakis, JN: Poly-N-acetyl Glucosamine: Will it Replace Cyanoacrylate for the Treatment of Gastric Varicies? A Pilot Study in a Rabbit Model. Endoscopy Journal, 30 #3, S41-42, 1998.

 

 

81.

Kulling, D., Vournakis, J., Woo, S., Demcheva, M., Tagge, D., Rios, G., Finkielsztein and Hawes, R.:  Endoscopic injection of bleeding esophageal varices with a poly-N-acetyl glucosamine gel formulation in the canine portal hypertension model. Gastrointestinal Endoscopy: 49, #6,764-771, 1999.

 

 

82.

Maitre, N., Brown, J.M., Demcheva, M., Kelley, J.E., Wilson, M.C., Vournakis, JN and Cole, D.J.: Primary T-Cell and Activated Macrophage Response Associated with Tumor Protection Using Peptide/Poly-N-Acetyl gluscosamine Vaccination. Clinical Cancer Res: 5, 1173-1182, 1999.

 

 

83.

Cole, D.J., Connolly, R.J., Chan, M.W., Schwaitzberg, S.D., Byrne, T.K., Adams, D.B., Baron, P.L., O’Brien, P.H., Metcalf, J.S., Finkielsztein, S., Demcheva, M. and Vournakis, JN: A Pilot Study Evaluating the Efficacy of a Fully Acetylated Poly-N-Acetyl Glucosamine Membrane Formulation as a Topical Hemostatic Agent. Surgery: 126, 510-517, 1999.

 

 

84.

Chan, M.W., Schwaitzberg, S.D., Demcheva, M., Finkielsztein, S., Vournakis,      JN  and Connolly, R.J. Comparison of Poly-N-acetyl Glucosamine (pGlcNAc) with Absorbable Collagen (Actifoam), and Fibrin Sealant (Bolheal) for Achieving Hemostasis in a Swine Model of Splenic Hemorrhage. J. Trauma: 48, 454-457, 2000.

 

 

85.

Kelley, J.R., Brown, J.B., Frasier, M.M., Baron, P.L., Schweinfest, C.W., Vournakis, JN, Watson, D.K. and Cole, D.J.: The Cancer Associated Sm-Like (CaSm) Oncogene: A Novel Target for Gene Therapy of Pancreatic Cancer, Surgery:128, 353-360, 2000.

 

 

86.

Nguyen, C., Bui, J., Demcheva, M., Vournakis, JN, Cole, D.J. and Gillanders, W.E., Sustained Release of GM-CSF Delivery from a Modular Peptide-Based Cancer Vaccine Alters Vaccine Microenvironment and Enhances Antigen-Specific T cell Response: J. Immunotherapy, 24: 420-429, 2001.

 

 

87.

Kelley JR, Fraser MM, Schweinfest CW, Vournakis, JN, Watson DK, Cole, DJ. Combinatorial CaSm Antisense/Gemcitabine chemo-gene therapy leads to prolonged survivorship in a murine model of pancreatic cancer. Surgery, 130: 280-288, 2001.

 

 

88.

Nguyen CL, Bui JT, Demcheva M, Vournakis JN, Cole DJ, Gillanders WE. Systemic IL-2 alters trafficking of antigen-specific of T cells and enchances the primary and memory response to peptide vaccination. Surgical Forum 52:286-88, 2001.

 

 

89.

Vournakis, JN, Demcheva, M., Whitson, A., Connolly, R., Cole, D., Valeri, C.R. and Finkielsztein, S.: Development and Properties of a New hemostatic Device for the Rapid Control of Hemorrhage in Combat Casualty Care: Naval Research Review, in press, 2002.

 

 

90.

Ikeda, Y., Young, L.H., Vournakis, JN and Lefer, A.M. Vascular Effects of Poly-N-Acetyl Glucosamine in Isolated Rat Aortic Rings: J. Surg. Res, 102: 215-220, 2002.

 

 

91.

Nguyen, C.L., Bui, J.T., Rubenstein, M.P., Demcheva, M., Vournakis, JN, Cole, D.J. and Gillanders, W.E., Mechanisms of Enhanced Antigen-specific T Cell Response Following Vaccination with a Novel Peptide-based Cancer Vaccine and Systemic Interleukin-2 (IL-2): J. Vaccine, in press, 2003.

 

 

92.

Vournakis, JN, Demcheva, M., Whitson, A.B., Finkielsztein, S. and Connolly, R.J., The RDH Bandage: Hemostasis and Survival in a Lethal Aortotomy Hemorrhage Model: J. Surgical Research. 113:1-5, 2003

 

 

93.

Salem, M.L., Kadima, A., Zhou, Y., Rubinstein, M.P., Demcheva, M., Vournakis, JN, Cole, D.J. and Gillanders, W.E. Paracrine Release of IL-12 Stimulates Interferon-gamma Production and Dramatically Enhances the Antigen-specific T-cell Response after Vaccination with a Novel Peptide-based Cancer Vaccine. J. Immunology. 172:5159-5167, 2004.

 

 

94.

Vournakis, JN, Demcheva, Whitson, A.M., Guirca, R. and Pariser, E.R. Isolation, Purification and Characterization of Poly-N-acetyl Glucosamine for use as a Hemostatic Agent. J. Trauma (2004) 57:S2-S6.

 

 

95.

An, Y.H., Hsu, Y.T., Mironov, V.M., Kang, Q.K., Brockbank, K.G., Atala, A.  and Vournakis J: Delivery of transfected cells for tumor embolization  A hypothesis. MUSC Orthop J (2004) 7:71-77.

 

 

96.

Kang, Q.K., Moreira, P.I., Demcheva, M., Vournakis, JN and An, Y.H. Arterial Embolization Using Poly-N-acetyl Glucosamine Gel in a Rat Kidney Model: Anatomical Research (2005) 284A: 454-459.

 

 

97.

Kang, Q.I., Hill, C.M., Demcheva, M.V., Vournakis, JN and An, Y.H. Poly-N-Acetyl Glucosamine-SO4 for Repairing Osteochondral Defects in Rabbitts. Key Engineering Materials Vols. 288-289 (2005), Trans Tech Publications, Ltd., Zurich, Switzerland, pgs. 83-86.

 

 

98.

Hill, C.M., An, Y.H., Kang, Q.K., Demcheva, M.V., Whitson, S.W. and Vournakis, JN Poly-N-Acetyl Glucosamine as a Scaffold for Cartilage Tissue Engineering in Nude Mice. Key Engineering Materials Vols. 288-289 (2005), Trans Tech Publications, Ltd., Zurich, Switzerland, pgs. 71-74.

 

 

99.

An, Y.H., Farino, M., Kang, Q.K., Demcheva, M.V. and Vournakis, JN Glucosamine for Preventing Bacterial Adhesion to Titanium Surfaces. Key Engineering Materials Vols. 288-289 (2005), Trans Tech Publications, Ltd., Zurich, Switzerland, pgs. 343-346.

 

 

100.

Fischer, T.H., Thatte, H.S., Nichols, T.C., Bender, D.E., Bellinger, D.A. and Vournakis, JN Synergistic Platelet Integrin Signaling and Factor XII Activation in Poly-N-acetyl Glucosamine Fiber Mediated Hemostasis. J Biomaterials (2005) 26: 5433-5443.

 

 

101.

Fischer, T.H., Bode, A.P., Demcheva, M. and Vournakis, JN Hemostatic Properties of Glucosamine-Based Materials. J. Biomedical Materials Research. (2006) 80A:167-174.

 

 

102.

Salem, M.L., Kadima, A.N., El-Naggar, S., Gillanders, W.E., Demcheva, M., Vournakis, JN and Cole, D.J. Novel non-viral delivery approaches for IL-12 protein and gene systems: curbing toxicity and enhancing adjuvant activity. J. Interferon & Cytokine Res. (2006) 26:593-608.

 

 

103.

Fischer, T.H., Smith, C.J. and Vournakis, J.V. Mechanisms for the Interaction of hemostatic Systems with Foreign Materials. Trends in Biomat. Res. (2006) 361-68.

 

 

104.

Pietramaggiori, G., Yang, H., Scherer, S.S., Kaipainen, A., Chan, R.K., Alperovich, M., Newalder, J., Demcheva, M., Vournakis, JN, Valeri, R.C., Hechtman, H.B., Orgill, D.P. Effects of poly-N-acetyl glucosamine (pGlcNAc) patch on wound healing in db/db mouse. J. Trauma (2008) 64(3):803-808.

 

 

105.

Vournakis, J., Eldridge, J., Demcheva M. and Muise-Helmericks, R. Poly-N-acetyl Glucosamine Nanofibers Regulate Endothelial Cell Movement and Angiogenesis:  Dependency on Integrin Activation of Ets1. J. Vascular Res. (2008) 45:222-232.

 

 

106.

Fischer, T.H., Valeri, C.R., Smith, C.J., Scull, C.M.,  Merricks, E.P., Nichols, T.P., Demcheva, M. and Vournakis, JN Non-classical Processes in Surface Hemostasis: Mechanisms for the Poly-N-acetyl glucosamine-induced Alteration of Red Blood Cell Morphology and Prothrombogenicity. J. Biomedical Materials Res. (2008) 3:1-9.

 

 

107.

Ehrlich, H., Janussen, D., Simon P., Bazhenov, V.V., Shapkin, N.P., Erler, C., Mertig, M., Born, R., Heinemann, S., Hanke, T., Worch, H., and Vournakis, JN Nanostructural Organization of Naturally Occurring Composites. Part II. Silica-Chitin-Based Biocomposites. (2008) J. Nanomat, in press.

 

 

108.

Smith, C.J., Vournakis, JN, Demcheva, M. and Fischer, T.H. Differential Effect of Materials for Surface Hemostasis on Red Blood Cell Morphology. Microscopic Res. & Techn. (2008) 71:721-729.

 

 

109.

Scherer, S.S., Pietramaggiori, G., Matthews, J., Perry, S., Assman, A., Carothers, A., Demcheva, M., Muise-Helmericks, R.C., Seth, A., Vournakis, JN, Valeri, R., Hechtman, H. and Orgill, D.P. Bioactive Poly-N-acetyl Glucosamine Nano-fibers: a New Approach to Treat Complicated Tissue Defects. Annals of Surgery (2009) 250(2):322-330.

 

 

110.

Fischer, T.H., Vournakis, JN, Manning, J.E., McCurdy, S.L., Rich, P.B., Nichols, T.C., Scull, C.M., McCord, M.G., Dacorta, J.A. and Smith, C.J. The Design and Testing of a Dual Fiber Textile Matrix for Accelerating Surface Hemostasis. J. Biomed. Mater. Res. B. Appl. Biomater. (2009) 91:381-389.

 

 

111.

Salem, M.L., Attia, W.Y., Al-Bolkiny, Y.E., Al-Sharkawi, I.M., Demcheva, M and Vournakis, J.N. Using Poly-N-acetyl Glucosamine Gel Matrix to Deliver IL-12 with Anti-Schistosomiasis Vaccination. J. Infect Dev Ctries (2010) 4(5):318-328.
   
112.

Salem, M.L. Demcheva, M., Gillanders, W.L, Cole, D.J. and Vournakis, J.N. Poly-N-acetyl Glucosamine Gel Matrix as a Non-Viral Delivery Vector for DNA-Based Vaccination. Anti-Cancer Res (2010)  30:3889-3894.

   
113.

Beeson, C.B., Beeson, G.C., Buff, H., Eldridge, J., Zhang, A., Seth, A., Demcheva, M., Vournakis, J.N. and Muise-Helmericks, R.C. Integrin-dependent Regulation of PGC-1 results in increased fatty acid oxidation. (2011) J. Vascular Res., in press.

   
114.

Lindner, H.B.,  Zhang, A., Eldridge, E.,  Demcheva, M., Tsichilis, P., Seth, A., Vournakis, J.N. and Muise-Helmericks, R.C. Anti-bacterial Effects of  Poly-N-acetyl Glucosamine Nanofibers in Cutaneous Wound Healing: Requirement for AKT1. PLoS1 (2011), in press.

   
115.

Vournakis, J.N., Fischer, T., Lindner, H.B., Demcheva, M., Seth, A. and Muise-Helmericks, R.C. Poly-N-acetyl Glucosamine Nanofibers Derived from a Marine Diatom: Applications in Diabetic Wound Healing and Tissue Regeneration. (2011), in Nanotechnology and Nanomedicine in Diabetes, in press.

   
116.

Valeri, C.R. and Vournakis, J.N. The mRDH Bandage for Surgery and Trauma: Data Summary and Comparative Review. J. Trauma (2011), in press.

   
117.

Muise-Helmericks, R.C., Demcheva, M., Vournakis, J.N. and Seth, A. Poly-N-acetyl Glucosamine Fibers Activate Bone Regeneration in a Rabbit Femur Injury Model. J. Trauma (2011), in press.

   
118.

Spinde. K., Kammer, M., Ehrlich, H., Vournakis, J.N. and Brunner, E. Biomimetic Silicification of Chitin. Chem. Of Mater. (2011), in press.

   
119.

Gorapalli, D., Seth, A., Vournakis, J., Whyne, C., Akens, M., Zhang, A., Demcheva, M. and Yee, A. Evaluation of a Novel Poly-N-acetyl Glucosamine (pGlcNAc) Hydrogel for the Treatment of Degenerating Intervertebral Disc. Spine (2011), submitted.

   

 

 

  

 

 

 

Educational Background

Research Interests

Chronology of Employment

Management and Administrative Experience

Biotechnology/Industry Board and Consulting Activities

Fellowships, Research Grants and Awards

Honors and Society Memberships

Other Professional Activities

Publications and Bibliography

 

 
 
 
 
 
 
 
 
Last updated: May 1, 2011